LOCUS       Exported                4013 bp ds-DNA    circular SYN 10-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0051max_producer_rhlI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Daniel Gerngross
  TITLE     piG0051max
  JOURNAL   Exported 10 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        50..84
                     /note="const. promoter (J23100)"
     RBS             87..91
                     /note="RBS (B0034)"
     CDS             98..700
                     /codon_start=1
                     /product="RhlI-LVA-Barcode"
                     /note="rhlI (C0070)"
                     /translation="MIELLSESLEGLSAAMIAELGRYRHQVFIEKLGWDVVSTSRVRDQ
                     EFDQFDHPQTRYIVAMSRQGICGCARLLPTTDAYLLKDVFAYLCSETPPSDPSVWELSR
                     YAASAADDPQLAMKIFWSSLQCAWYLGASSVVAVTTTAMERYFVRNGVILQRLGPPQKV
                     KGETLVAISFPAYQERGLEMLLRYHPEWLQGVPLSMAV"
     terminator      718..804
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      919..1690
                     /note="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1691..2353
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /note="pBBR1 Rep"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     oriT            2364..2472
                     /note="incP origin of transfer"
     CDS             complement(2618..3808)
                     /note="TetR"
     terminator      3915..4009
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 actagtcgcc agggttttcc cagtcacgac gcggccgcaa gcttgcatgt tgacggctag
       61 ctcagtccta ggtacagtgc tagctaagga ggtatcaatg atcgaactgc tgtccgaatc
      121 cctggaaggt ctgtccgctg ctatgatcgc tgaactgggt cgttaccgtc accaggtttt
      181 catcgaaaaa ctgggttggg acgttgtttc cacctcccgt gttcgtgacc aggagttcga
      241 ccagttcgac cacccgcaga cccgttacat cgttgctatg tcccgtcagg gtatctgcgg
      301 ttgcgctcgt ctgctgccga ccaccgacgc ttacctgctg aaagacgttt tcgcttacct
      361 gtgctccgaa accccgccgt ccgacccgtc cgtttgggaa ctgtcccgtt acgctgcttc
      421 cgctgctgac gacccgcagc tggctatgaa aatcttctgg tcctccctcc agtgcgcttg
      481 gtacctgggt gcttcctccg ttgttgctgt taccaccacc gctatggaac gttacttcgt
      541 tcgtaacggt gttatcctcc agcgtctggg tccgccgcag aaagttaaag gtgaaaccct
      601 ggttgctatc tccttcccgg cttaccagga acgtggtctg gaaatgctgc tgcgttacca
      661 cccggaatgg ctccagggtg ttccgctgtc catggctgtt taataaatta aaggcatcaa
      721 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      781 aacgctctcc tgagtaggac aaatccgccg ccctagacag ctgggcgcgc cccccctacg
      841 ggcttgctct ccgggcttcg ccctgcgcgg tcgctgcgct cccttgccag cccgtggata
      901 tgtggacgat ggccgcgagc ggccaccggc tggctcgctt cgctcggccc gtggacaacc
      961 ctgctggaca agctgatgga caggctgcgc ctgcccacga gcttgaccac agggattgcc
     1021 caccggctac ccagccttcg accacatacc caccggctcc aactgcgcgg cctgcggcct
     1081 tgccccatca atttttttaa ttttctctgg ggaaaagcct ccggcctgcg gcctgcgcgc
     1141 ttcgcttgcc ggttggacac caagtggaag gcgggtcaag gctcgcgcag cgaccgcgca
     1201 gcggcttggc cttgacgcgc ctggaacgac ccaagcctat gcgagtgggg gcagtcgaag
     1261 ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg gttccaaggg
     1321 ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc cccggagggg
     1381 ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc aggggtgccc
     1441 ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa aaatcaacaa
     1501 cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag ctcattgcgt
     1561 aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt gttgtcgcgc
     1621 tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc cctaccgcat
     1681 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg
     1741 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa
     1801 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt
     1861 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata
     1921 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg
     1981 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt
     2041 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct
     2101 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc
     2161 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct
     2221 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc
     2281 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtaacgct
     2341 gccgcgccgg tagggccggc ctacggccag cctcgcagag caggattccc gttgagcacc
     2401 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
     2461 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
     2521 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
     2581 ccgaagcagg gttatgcagc ggaaaaggac aattgtctca ggtcgaggtg gcccggctcc
     2641 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg
     2701 ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag
     2761 tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt
     2821 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
     2881 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
     2941 ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     3001 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     3061 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     3121 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gccacaatgg
     3181 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggac
     3241 ggcgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     3301 tgagcaccgc cgccgcaagg aatggtgcgt gcagggagat ggcgcccaac agtcccccgg
     3361 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     3421 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     3481 cggtgatgcc ggccacgatg cgtccggcgt agagaatcca caggacgggt gtggtcgcca
     3541 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa
     3601 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg
     3661 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc
     3721 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
     3781 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
     3841 gtgataaact accgcattac agtttatcga tgataagctg tcaaatttaa atcgtaatta
     3901 ttggggaccc ctggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     3961 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aag
//
